In vitro evaluation of commercial probiotic products used for marine shrimp cultivation in Thailand

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In vitro evaluation of commercial probiotic products used for marine shrimp cultivation in Thailand

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The objective of this study was to evaluate the effectiveness of 12 commercial probiotic products in Thailand, used in marine shrimp cultivation, based on two criteria namely, accuracy of the information on product labels as regards the number and types of microorganisms and acceptability of the number of probiotic microorganisms at 106 colony forming unit (CFU)/g in the products. Of the 12 products sampled, only two of them provided adequate information on the number and composition of microorganisms and their proper dosage. In addition, none of the probiotic products possessed the correct number and composition of microorganisms or qualitative extracellular enzymes, declared on their labels nor did they show any in vitro inhibitory activity on shrimp pathogenic Vibrio harveyi. However, a few products were capable of biosynthesis of amylase, protease and lipase with high capacities.

Table 1. Number of total heterotrophic bacteria of tested commercial probiotic products.

Commercial

probiotic

products

 

Country

of origin

 

Measured

total aerobic

heterotrophic

bacteria (CFU/g)

Labeled

total aerobic

heterotrophic

bacteria (CFU/g)

Product 1

Product 2

Product 3

Product 4

Product 5

Product 6

Product 7

Product 8

Product 9

Product 10

Product 11

Product 12

Thailand

Thailand

China

Thailand

China

Thailand

Thailand

Thailand

Thailand

Thailand

US

Thailand

8.57 x 104

2.07 x 104

1.26 x 104

8.23 x 103

4.43 x 103

6.13 x 102

2.50 x 105

1.26 x 108

7.55 x 107

1.70 x 107

1.30 x 109

1.10 x 106

109

109

109

109

109

109

ND

ND

109

ND

109

ND

Table 2. Species composition of bacteria in tested commercial probiotic products.

Table 3. Species of Bacillus in tested commercial probiotic products.

The present investigation suggests that only two of the 12 tested commercial probiotic products (product 9 and 11) contained the numbers of probiotic microorganisms recommended for shrimp cultivation and provided the correct label containing information on product number as well as composition of microorganisms. Furthermore, some of the tested commercial probiotic products had high capacity for amylase, protease and lipase production and none showed any in vitro inhibitory activity against V. harveyi. The effectiveness of the tested commercial probiotic products in the present study was determined under in vitro conditions, however, the effectiveness of probiotic products when used in situations directly relevant to aquaculture condition may not be consistent with in vitro conditions.

Subuntith Nimrat1 and Verapong Vuthiphandchai2

1Department of Microbiology and Environmental Science Program, Faculty of Science, Burapha University, Chon Buri, 20131, Thailand.

2Department of Aquatic Science, Faculty of Science, Burapha University, Chon Buri, 20131, Thailand.

Source: African Journal of Biotechnology Vol. 10(22), pp. 4643-4650, 30 May, 2011

Available online at http://www.academicjournals.org/AJB

DOI: 10.5897/AJB10.2404

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